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Bone, shell, and sediment studies have contributed much to the paleontological record, including that relating to hominoids.

Strong acidity and mild to strong alkalinity induce greatly increased racemization rates.

This includes racemization rate variation among species and organs, and is affected by the depth of decomposition, porosity, and catalytic effects of local metals and minerals.

Conventional racemization analysis tends to report a D-alloisoleucine / L-isoleucine (A/I or D/L ratio).

The amino acid derivative hydrolysis product can be combined with a chiral specific fluorescent, separated by chromatography or electrophoresis, and the particular amino acid D: L ratio determined by fluorescence.